Website Going down due to max pool size issue

We are using Nopcommerce 4.40 and facing the following error:

Timeout expired. The timeout period elapsed prior to obtaining a connection from the pool. This may have occurred because all pooled connections were in use and max pool size was reached.

We generally face this error when there are around 90-100 concurrent users on our site.

You can increase your db connection pool size by adding ;Min Pool Size=100;Max Pool Size=2024;Pooling=true; to your db connection string

Already Tried this but still getting the same error.