Just to be certain we have hit all points, I have had the same problem and the solution for my situation was to add System.Web.Ajax to my Bin folder. Hope this helps somebody.
I agree with WaltDJr. His solution solved my problem after a day of banging my head against the wall.
I uploaded my app to my GoDaddy hosting server running Plesk Panel 9.5.2 and all of the scripts on the app stopped working. After digging, I found I was getting an error "asp.net ajax client-side framework failed to load". A day of Googling and trying a dozen different solutions didn't fix the problem.
Then, my savior, WaltDJr, came along.
Thanks WaltDJr. I opened IIS on my server, opened Handler Mappings for my app, clicked on "revert to parent" and my problem was solved. Big heavy sighhhh......